Regulatory

Part:BBa_K3443002

Designed by: Christoph Martin Sannwald   Group: iGEM20_UUlm   (2020-09-14)


PthlA constitutive promotor from C. acetobutylicum

PthlA is a constitutive promotor fund in Clostridium acetobutylicum. It can be used in artificial acetone synthesis plasmids. Here it regulates the expression of genes: thlA, ctfA/ctfB and adc.


Improvement from NJTch_China 2022

Document

Results:

The fluorescence intensity shows that the promoter thlF7 has a trends in aerobic inhibition. But the performence in microaerobic situation is also inhibited.

Fig.2. Expression effect of PthlA and PthlAF7-bs2 at different oxygen concentrations

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Parameters
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